Studies on Transition 
 in -  Diplacus  - PLANTS
 

FRAGARIA STRAWBERRY

Strawberry MYB10 is the main factor responsible for the pigmentation in the skin and flesh of the berries. Notably, it contains a string of polyadenine-thymine (polyTA) in the second intron. Interestingly, the three taxa—Fragaria moschata, Fragaria vesca, and the cultivated plant Fragaria x ananassa—each have polyTA strings of different lengths. However, they all share the same exon and intron information, differing only in the lengths of their polyTA strings. See the picture below for reference.


Fragaria x anannassa STR polyTA length distribution in the first intron of MYB10 

N

%

Length

SRX25741866

 4

7

32-33

> 100 GB

13

22

31

2024

 42

71

29-30

PacBio SMRT™


Fragaria moschata STR polyTA length distribution in the first intron of MYB10 (EU155163.1)


N

%

Length

 ERX12134001

2  

15-16 TA 

29/20 GB, 4-30 kb strl

40

 42

14 TA 

2024

 53 

58

13-14TA 

PacBio SMRT™





The asymmetric distribution of polyTA in short tandem repeats (STRs) as somatic mutations suggests that STRs tend to shorten. The somatic mutation rate is notably high (p > .65). Use BLAST EU155163   against ERX12134001 to gain an overview.

You achieve better results when you omit one TA repeat from the STR.

The result along the STR will resemble what is depicted above. 

Fragaria vesca  EU155163.1   ERX12326309


N

%

Length TA

ERX12326309

 65

78

18-19

24/9 GB

 17

20

17

2-20 k

2

2

15-16

2024

The unequal distribution of polyTA in short tandem repeats (STRs) as somatic mutations indicates a tendency for STRs to lengthen.