Transition studies 

 in plants             by Rolf Baumberger 

 PROSPECTS

The past year was very productive for us.
On the one hand,  the genomes (47) of almost all Diplacus varieties are now available online: https://blast.ncbi.nlm.nih.gov/Blast.cgi. (e.g. SRX6077453)
This genome information benefits our search for more like functional genomics, which will be the basics to understand a plant living system evolutionarily.
On the other hand, several Diplacus plants in transition zones (formerly hybrid zones, e.g., at Santa Susana Pass) have also been labeled and sequenced and have already been statistically analyzed see:https://academic.oup.com/evlett/article/7/5/293/7190212
The conditions are excellent for our needs. A plant in transition not only changes its phenotype but also its genotype at the same time. Thus, such marked plants can be genomically analyzed again after about four years, and the possible difference is what has changed in the genome in the meantime. 

- We are focusing primarily on altering short tandem reads within the introns.



We are thinking in particular of our T-plants T27 & T30. We still have frozen collected leaf material from these plants from 20 years ago and now have cloned plants in our greenhouse for genomic comparison.


In addition, the red-flowering Santa Ana variety


and the red-flowering Diplacus 

plants on


Santa Catalina Island should also be genomically analyzed to complete the genome collection.

Some form of support would be helpful. Especially needed are some genomic sequencing (2x250bp) of the forms mentioned above.
We would like to know if we can find someone to support us beneficially or sequence genomes in his lab. The results will be published together later.

N.B.
see: "ColdSpringReview-Speciation"

We are glad to see this mentioned in this recent publication about "speciation." The insight into those genomic structures will spur new theories. However, after all, it will validate the old theories. - A quick paradigm shift could happen in a very short time.
We like the expression "evolutionary forces".

02-16-24 rolfy